Database : HANSEN Search on : PROTEINAS DE BACTERIAS [Subject descriptor] References found : 35 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 13719 Author: Kang, T. -J; Kim, S. -K; Lee, S. -B; Chae, G. -T; Kim, J. -P Title: Comparison of two different PCR amplification products (the 18-kDa protein gene vs. RLEP repetitive sequence) in the diagnosis of Mycobacterium leprae ..- Source: s.l; s.n; 2003. 5 p. tab. Abstract: To determine the best molecular method for diagnosing leprosy, two sets of Mycobacterium leprae-specific primers were compared. Fresh biopsies and slit skin smear samples were obtained from 67 leprosy patients and examined by touchdown (TD) PCR using primers amplifying either a 129-bp fragment of the RLEP repetitive sequence or a 360-bp fragment of the 18-kDa protein gene of M. leprae. Seventeen of 30 (56.7 per cent) biopsy specimens and four of 37 (10.8 per cent) slit skin smear specimens were positive using the primer for the 18-kDa protein gene, whereas 24 of 30 (80 per cent) biopsy and 27 of 37 (73 per cent) slit skin smear samples showed detectable PCR products in the RLEP repetitive sequence. Twenty-one of 31 cases (67.7 per cent) with a bacterial index of zero were PCR positive for the primer RLEP repetitive sequence. These results demonstrate that detection of M. leprae using PCR with primers to a RLEP sequence is more sensitive and specific than PCR with the 18-kDa protein gene primers and also slit smears with acid fast staining. PCR of RLEP repetitive sequences is therefore a useful means of detecting M. leprae DNA even when it is present at very low levels. (AU). Descriptors: ANTICORPOS ANTIBACTERIAS/sangue PROTEINAS DE BACTERIAS/genet HANSENIASE/diag HANSENIASE/microbiol MYCOBACTERIUM LEPRAE/genet MYCOBACTERIUM LEPRAE/isol REACAO EM CADEIA DA POLIMERASE/métodos PELE/microbiol - SEQUÊNCIAS REPETITIVAS DO ACIDO NUCLEICO  PRIMERS DO DNA Limits: ESTUDO COMPARATIVO HUMANO Electronic Medium: http://www.ilsl.br Location: BR191.1; 09117/s

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Id: 13639 Author: Kang, T. -J; Kim, S. -K; Lee, S. -B; Chae, G. -T; Kim, J. -P Title: Comparison of two different PCR amplification products (the 18-kDa protein gene vs. RLEP repetitive sequence) in the diagnosis of Mycobacterium leprae ..- Source: s.l; s.n; 2003. 5 p. ilus, tab. Abstract: To determine the best molecular method for diagnosing leprosy, two sets of Mycobacterium leprae-specific primers were compared. Fresh biopsies and slit skin smear samples were obtained from 67 leprosy patients and examined by touchdown (TD) PCR using primers amplifying either a 129-bp fragment of the RLEP repetitive sequence or a 360-bp fragment of the 18-kDa protein gene of M. leprae. Seventeen of 30 (56.7 per cent) biopsy specimens and four of 37 (10.8 per cent) slit skin smear specimens were positive using the primer for the 18-kDa protein gene, whereas 24 of 30 (80 per cent) biopsy and 27 of 37 (73 per cent) slit skin smear samples showed detectable PCR products in the RLEP repetitive sequence. Twenty-one of 31 cases (67.7 per cent) with a bacterial index of zero were PCR positive for the primer RLEP repetitive sequence. These results demonstrate that detection of M. leprae using PCR with primers to a RLEP sequence is more sensitive and specific than PCR with the 18-kDa protein gene primers and also slit smears with acid fast staining. PCR of RLEP repetitive sequences is therefore a useful means of detecting M. leprae DNA even when it is present at very low levels. (AU). Descriptors: ANTICORPOS ANTIBACTERIAS/sangue PROTEINAS DE BACTERIAS/genet PRIMERS DO DNA HANSENIASE/diag HANSENIASE/microbiol MYCOBACTERIUM LEPRAE/genet MYCOBACTERIUM LEPRAE/isol REACAO EM CADEIA DA POLIMERASE/métodos SEQUÊNCIAS REPETITIVAS DO ACIDO NUCLEICO PELE/microbiol Limits: ESTUDO COMPARATIVO HUMANO Electronic Medium: http://www.ilsl.br Location: BR191.1; 09054/s

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Id: 13387 Author: Khanolkar, S. R Title: Preliminary studies of the metabolic activity of purified suspensions of Mycobacterium leprae ..- Source: s.l; s.n; feb. 1982. 3 p. graf. Abstract: Mycobacterium leprae isolated from armadillo tissue incorporated radioactivity from D-(14C)glucose and (14C)protein hydrolysate. In the presence of glucose, the rate of incorporation of (14C)protein hydrolysate was increased. Uptake of glucose was inhibited by 2-deoxy-D-glucose and sodium azide; that of the amino acids was inhibited by puromycin and chloramphenicol and, weakly, by cycloheximide.(AU). Descriptors: AMINOACIDOS/metab PROTEINAS DE BACTERIAS/bios CINETICA TRANSPORTE BIOLOGICO AZIDA SODICA PUROMICINA/farmacol MYCOBACTERIUM LEPRAE/metab CLORANFENICOL/farmacol Limits: SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 00904/s

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Id: 12241 Author: Chae, Gue-Tae; Kim, Min-Joo; Kang, Tae-Jin; Lee, Seong-Beom; Shin, Hang-Kye; Kim, Jong-Pill; Ko, Young-Hoon; Kim, Sung-Hwa; Kim, Nan-Hee Title: DNA-PCR and RT-PCR for the 18-kDa gene of mycobacterium leprae to asses the efficacy of multi-drug therapy for leprosy ..- Source: s.l; s.n; 2002. 6 p. tab, graf. Descriptors: PROTEINAS DE BACTÉRIAS BIOPSIA DNA BACTERIANO DENSITOMETRIA QUIMIOTERAPIA COMBINADA ELETROFORESE EM GEL DE AGAR GLICOLIPIDIOS GLICOLIPIDIOS LEPROSTATICOS HANSENIASE HANSENIASE ESTUDOS LONGITUDINAIS CAMUNDONGOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE REAÇAO EM CADEIA POR POLIMERASE RNA MENSAGEIRO REPRODUTIBILIDADE DE RESULTADOS Location: BR191.1; 08644/s

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Id: 12234 Author: Ohyama, Hideki; Matsushita, Sho; Nishimura, Fusanori; Kato, Nahoko; Hatano, Kentaro; Takashiba, Shogo; Murayama, Yoji Title: T cell responses to major membrane protein II (MMP II) of mycobacterium leprae are restricted by HLA-DR molecules in patients with leprosy ..- Source: s.l; s.n; 2001. 8 p. tab, graf. Descriptors: SEQUENCIA DE AMINOACIDOS PROTEINAS DE BACTÉRIAS ANTIGENOS HLA-DR INTERFERON TIPO II INTERLEUCINA-5 HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE LINFOCITOS T Location: BR191.1; 08636/s

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Id: 11944 Author: Visca, Paolo; Fabozzi, Giulia; Petrucca, Andrea; Ciaccio, Chiara; Coletta, Massimo; De Sanctis, Giampiero; Bolognesi, Martino; Milani, Mario; Ascenzi, Paolo Title: The truncated hemoglobin from Mycobacterium leprae ..- Source: s.l; s.n; 2002. 7 p. ilus, graf. Descriptors: MYCOBACTERIUM LEPRAE SEQUENCIA DE AMINOACIDOS PROTEINAS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS SEQUENCIA DE BASES CLONAGEM MOLECULAR HEMOGLOBINAS HEMOGLOBINAS HEMOGLOBINAS LIGANTES MODELOS MOLECULARES DADOS DE SEQUENCIA MOLECULAR PROTEINAS RECOMBINANTES PROTEINAS RECOMBINANTES HOMOLOGIA DE SEQUENCIA DE AMINOACIDOS Location: BR191.1; 08601/s

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Id: 11939 Author: Meada, Yumi; Makino, Masahiko; Crick, Dean C; Mahapatra, Sebabrata; Srisungnam, Sopa; Takii, Takemasa; Kashiwabara, Yoshiko; Brennan, Partrick J Title: Novel 33-kilodalton lipoprotein from Mycobacterium leprae ..- Source: s.l; s.n; 2002. 6 p. ilus, graf. Descriptors: MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MONOCITOS ANTIGENOS DE BACTÉRIAS ANTIGENOS DE BACTÉRIAS ANTIGENOS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS CLONAGEM MOLECULAR ESCHERICHIA COLI EXPRESSAO GENICA GENES BACTERIANOS INTERLEUCINA-12 PESO MOLECULAR ANALISE SEQUENCIAL PRECURSORES DE PROTEINAS PRECURSORES DE PROTEINAS PRECURSORES DE PROTEINAS Location: BR191.1; 08605/s

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Id: 11757 Author: Chae, Gue-Tae; Kim, Min-Joo; Kang, Tae-Jin; Lee, Seong-Beom; Shin, Hang-Kye; Kim, Jong-Pill; Ko, Young-Hoon; Kim, Sung-Hwa; Kim, Nan-Hee Title: DNA-PCR and RT-PCR for the 18-KDa gene of Mycobacterium leprae to asses the efficacy of multi-drug therapy for leprosy ..- Source: s.l; s.n; 2002. 6 p. tab. Descriptors: PROTEINAS DE BACTÉRIAS BIOPSIA DNA BACTERIANO DENSITOMETRIA QUIMIOTERAPIA COMBINADA ELETROFORESE EM GEL DE AGAR GLICOLIPIDIOS LEPROSTATICOS HANSENIASE HANSENIASE ESTUDOS LONGITUDINAIS CAMUNDONGOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE REAÇAO EM CADEIA POR POLIMERASE RNA MENSAGEIRO REPRODUTIBILIDADE DE RESULTADOS IMUNOGLOBULINA M/BL CAMUNDONGOS REACAO EM CADEIA POR POLIMERASE VIA TRANSCRIPTASE CAMUNDONGOS NUS REACAO EM CADEIA POR POLIMERASE VIA TRANSCRIPTASE REVERSA Limits: ANIMAL Location: BR191.1; 08517/s

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Id: 11610 Author: Wilkinson, Robert John; Wilkinson, Katalin Andrea; Jurcevic, Stipo; Hills, Adrian; Sinha, Sudhir; Sengupta, Utpal; Lockwood, Diana N. J; Katoch, Kiran; Altman, Daniel; Ivanyi, Jurai Title: Specificity and function of immunogenic peptides from the 35-kilodalton protein of mycobacterium leprae ..- Source: s.l; s.n; 1999. 4 p. tab, graf. Descriptors: COMPLEXO MYCOBACTERIUM AVIUM MYCOBACTERIUM LEPRAE ANTIGENOS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS ANTIGENOS HLA-DR Location: BR191.1; 08433/s

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Id: 10362 Author: Offung, Fredrik; Lundin, Knut E. A; Meloen, Robert; Mustafa, Abu Salim Title: Human T cell recognition of the Mycobacterium leprae LSR antigen: epitopes and HLA restriction ..- Source: s.l; s.n; 1999. 9 p. tab, graf. Descriptors: SEQUENCIA DE AMINOACIDOS ANTIGENOS DE BACTÉRIAS ANTIGENOS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS EPITOPOS DE LINFOCITO T ANTIGENOS HLA TESTE DE HISTOCOMPATIBILIDADE IMUNIZAÇAO IMUNOFENOTIPAGEM HANSENIASE TRANSFORMAÇAO LINFOCITICA DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE PEPTIDIOS PEPTIDIOS PEPTIDIOS PROTEINAS RECOMBINANTES DE FUSAO PROTEINAS RECOMBINANTES DE FUSAO LINFOCITOS T Limits: ANIMAL Location: BR191.1; 07494/s

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Id: 10298 Author: Weir, R. E; Brennan, P. J; Butlin, C. R; Dockrell, H. M Title: Use of a whole blood assay to evaluate in vitro T cell responses to new leprosy skin test antigens in leprosy patients and healthy subjects ..- Source: s.l; s.n; 1999. 7 p. tab. Descriptors: ANTIGENOS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS INTERFERON TIPO II HANSENIASE HANSENIASE TRANSFORMAÇAO LINFOCITICA PESO MOLECULAR MYCOBACTERIUM LEPRAE TESTES CUTANEOS ESTERILIZAÇAO LINFOCITOS T Location: BR191.1; 07400/s

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Id: 10215 Author: Singh, Satish; Jenner, Peter J; Narayan, N. P. Shanker; Ramu, Gopal; Colston, M. Joseph; Prasad, H. Krishna; Nath, Indira Title: Critical residues of the mycobacterium leprae LSR recombinant protein discriminate clinical activity in erythema nodosum leprosum reactions ..- Source: s.l; s.n; 1994. 4 p. tab, graf. Descriptors: SEQUENCIA DE AMINOACIDOS ANTICORPOS ANTIBACTÉRIAS ANTIGENOS DE BACTÉRIAS LINFOCITOS B PROTEINAS DE BACTÉRIAS ESTUDOS DE CASOS E CONTROLES MAPEAMENTO DE EPITOPOS EPITOPOS ERITEMA NODOSO HANSENIASE LEPROMATOSA DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE FRAGMENTOS DE PEPTIDIOS PROTEINAS RECOMBINANTES DE FUSAO Location: BR191.1; 06916/s

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Id: 10166 Author: Struyk, Linda; Hawes, Gail E; Haanen, John B. A. G; Vries, Rene R. P. de; Van den Elsen, Peter J Title: Clonal dominance and selection for similar complementarity determining region 3 motifs among T lymphocytes responding to the HLA-DR3-associated Mycobacterium leprae shock protein 65-kd peptide 3-13 ..- Source: s.l; s.n; 1995. 8 p. ilus, tab. Descriptors: HANSENIASE TUBERCULOIDE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE LINFOCITOS T SEQUENCIA DE AMINOACIDOS PROTEINAS DE BACTÉRIAS SEQUENCIA DE BASES LINHAGEM CELULAR CHAPERONINAS CHAPERONINAS PRIMERS DO DNA ANTIGENO HLA-DR3 REGIAO VARIAVEL DE IMUNOGLOBULINA DADOS DE SEQUENCIA MOLECULAR FRAGMENTOS DE PEPTIDIOS FRAGMENTOS DE PEPTIDIOS RECEPTORES PARA ANTIGENOS DE CÉLULAS T ALFA-BETA Limits: HUMANO Location: BR191.1; 07079/s

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Id: 10145 Author: Colston, M. J Title: The cellular and molecular basis of immunity againts mycobacterial diseases ..- Source: s.l; s.n; 1996. 7 p. ilus. Descriptors: ADJUVANTES IMUNOLOGICOS ANTIGENOS CD VACINA BCG PROTEINAS DE BACTÉRIAS CITOCINAS IMUNIDADE ATIVA IMUNIDADE CELULAR HANSENIASE HANSENIASE LISOSSOMOS MACROFAGOS MYCOBACTERIUM AVIUM MYCOBACTERIUM BOVIS MYCOBACTERIUM LEPRAE MYCOBACTERIUM TUBERCULOSIS FAGOCITOSE FAGOSSOMOS LINFOCITOS T TUBERCULOSE TUBERCULOSE VACINAS Location: BR191.1; 07119/s

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Id: 10093 Author: Deshpande, Rajashri G; Khan, Mahfuz B; Bhat, Deepashree A; Navalkar, R. G Title: Immunoaffinity chromatographic isolation of a high molecular weight seroreactive protein from Mycobacterium leprae cell sonicate ..- Source: s.l; s.n; 1995. 7 p. ilus, tab. Descriptors: HANSENIASE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM PARATUBERCULOSIS ANTICORPOS MONOCLONAIS ANTIGENOS DE BACTÉRIAS ANTIGENOS DE BACTÉRIAS ANTIGENOS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS CITOCROMO B FERRITINA DADOS DE SEQUENCIA MOLECULAR PESO MOLECULAR HOMOLOGIA DE SEQUENCIA DE AMINOACIDOS Limits: ANIMAL HUMANO COELHOS Location: BR191.1; 07152/s

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Id: 10047 Author: Mitra, D. K; Rajalingam, R; Taneja, V; Bhattacharyya, B. C; Mehra, N. K Title: HLA-DR polymorphism modulates the cytokine profile of Mycobacterium leprae HSP-reactive CD4+ T cells ..- Source: s.l; s.n; 1997. 8 p. tab, graf. Descriptors: ADJUVANTES IMUNOLOGICOS PROTEINAS DE BACTÉRIAS LINFOCITOS T CD4-POSITIVOS CHAPERONINAS ANTIGENOS HLA-DR PROTEINAS DO CHOQUE TÉRMICO HANSENIASE MYCOBACTERIUM LEPRAE MYCOBACTERIUM TUBERCULOSIS POLIMORFISMO (GENÉTICA) CÉLULAS TH1 CÉLULAS TH2 Location: BR191.1; 07012/s

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Id: 10008 Author: Adams, E; Britto, W; Morgan, A; Sergeantson, S; Basten, A Title: Individuals from different populations identify multiple and diverse T-cell determinants on mycobacterial HSP70 ..- Source: s.l; s.n; 1994. 9 p. tab, graf. Descriptors: SEQUENCIA DE AMINOACIDOS VACINA BCG PROTEINAS DE BACTÉRIAS RAÇA CAUCASOIDE EPITOPOS ANTIGENOS HLA-DR PROTEINAS DO CHOQUE TÉRMICO HANSENIASE LEUCOCITOS MONONUCLEARES DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE NEPAL FRAGMENTOS DE PEPTIDIOS PROTEINAS RECOMBINANTES LINFOCITOS T TUBERCULOSE Location: BR191.1; 06904/s

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Id: 10001 Author: Vega-Lopez, F; Brooks, L. A; Dockrell, H. M; Smet, K. A. L. de; Thompson, J. K; Hussain, R; Stoker, N. G Title: Sequence and immunological characterization of a serine-rich antigen from mycobacterium leprae ..- Source: s.l; s.n; 1993. 9 p. ilus, tab, graf. Descriptors: SEQUENCIA DE AMINOACIDOS SEQUENCIA DE BASES ANTICORPOS ANTIBACTÉRIAS ANTIGENOS DE BACTÉRIAS ANTIGENOS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS CLONAGEM MOLECULAR GENES ESTRUTURAIS BACTERIANOS HANSENIASE LEPROMATOSA DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM TUBERCULOSIS OLIGODESOXIRRIBONUCLEOTIDIOS PROTEINAS RECOMBINANTES DE FUSAO MAPEAMENTO POR RESTRIÇAO ALINHAMENTO DE SEQUENCIA HOMOLOGIA DE SEQUENCIA DE AMINOACIDOS SERINA Location: BR191.1; 06897/s

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Id: 9995 Author: Launois, P; Niang, M'B. N'Diaye; Sarthou, J.-L; Rivier, F; Drowart, A; Van Vooren, J.-P; Millan, J; Huygen, K Title: T-cell stimulation with purified mycobacterial antigens in patients and healthy subjects infected with mycobacterium leprae: secreted antigen 85 is another immunodominant antigen ..- Source: s.l; s.n; 1993. 10 p. ilus, tab. Descriptors: ANTIGENOS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS PROTEINAS DO CHOQUE TÉRMICO INTERFERON TIPO II HANSENIASE TRANSFORMAÇAO LINFOCITICA MYCOBACTERIUM BOVIS MYCOBACTERIUM LEPRAE LINFOCITOS T Location: BR191.1; 06639/s

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Id: 9944 Author: Ilangumaran, S; Narayan, Shanker N. P; Ramu, G; Muthukkaruppan, V. R Title: Cellular and humoral immune responses to recombinant 65-kD antigen of Mycobacterium leprae in leprosy patients and healthy controls ..- Source: s.l; s.n; 1994. 7 p. tab, graf. Descriptors: HANSENIASE LEPROMATOSA HANSENIASE TUBERCULOIDE MYCOBACTERIUM BOVIS MYCOBACTERIUM LEPRAE ANTICORPOS ANTIBACTÉRIAS ANTIGENOS DE BACTÉRIAS PROTEINAS DE BACTÉRIAS PROTEINAS DO CHOQUE TÉRMICO IGG INTERLEUCINA-2 TRANSFORMAÇAO LINFOCITICA PROTEINAS RECOMBINANTES Location: BR191.1; 06847/s

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